Glacios 2 Cryo-TEM

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Université McGill – Faculty for Electron Microscopy Research

Manufacturer and model : Thermo Scientific Glacios 2 Cryo-TEM

External website : FEMR

Contact person : Angel Valdez

Part of RQMÉM : no

Address : Strathcona Anatomy & Dentistry Building, 3640, University Street, Montreal, QC H3A 0C7

High resolution and high throughput

Detectors, cameras and spectrometers :

  • direct electron detector Falcon 4i

  • Fringe-free imaging (FFI)

  • Aberration-Free Image Shift (AFIS)

  • Ceta-D camera with MicroED capacity

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Specifications :

  • E-CFEG : low-energy-spread cold field emission gun

  • Selectris energy filter

  • Automatic charger that can hold up to 12 automatic grids

  • Smart EPU software with EPU Multigrid

  • 200 kV accelerating voltage

Applications :

A flexible platform, idela for multiple applications including single particle analysis (SPA), cryo-electron tomography (cryo-ET) or micro-electron diffraction (MicroED). Cryo-microscopy with near-atomic resolution. Large area data acquisition without image distortion, structural characterization of micro- and nanocrystals.

Single particle analysis : structural determination of near-atomic resolution, samples can be studied directly in solution withtout the need for crystallisation.

Micro-electron diffraction (microED) : High resolution fast imagery with near atomic details extracted from individual nanocrystals smaller than 200 nm, even in a heterogeneous mixture.

With the Selectris energy filter and E-CFEG gun, we minimize impact of environmental factors and maximise system stability. E-CFEG gun allows a low energy spread, improved contrast with high spatial frequencies, for a better resolution in shorter time.

Smart EPU software with EPU Multigrid allows data collection over many days to maximize the microscope efficiency with automatic acquisitions on multiple grids.

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